生物油脂高产菌株筛选方法研究

Research of Screening Method of the High Bio-oil Production Microorganisms

本文采用脂肪粒计数法、苏丹III菌泥染色法、碳饥饿检出法等对生物油脂高产菌株进行了筛选比较研究。通过试验表明,苏丹III菌泥染色法简便快捷,其结果与菌体的油脂含量有较好的相关性,此法是筛选产脂菌株的较理想的初筛方法。碳饥饿检出法准确性较高,但过于繁琐;相比之下,脂肪粒计数法虽简便,但缺乏准确性,使用时必须同时考虑脂肪粒的大小。通过筛选,获得了一株生物油脂高产菌株IFFI01368,其菌体中油脂含量为54.25%。

Fat Particles Counting（FPC）, Yeast Mud Dying by Sudan Ⅲ（YMDSⅢ）, Culture of Lacking Carbon（CLC） were compared and approached in the essay. The results show that YMDSIII is simple and direct, its results are better interrelated with oil-producing quantity of yeast. This method is a relatively ideal screening method on choosing oil-producing microorganisms. CLC is relatively accurate, but it is too tedious By comparison, FPC is simple and convenient, but it is short of accuracy. When it is used, it must consider the size of fat particles. By screening, cellular oil content reached 54.25% （W/W）.