远志内生真菌抑菌活性筛选

Inhibitory Activity Screening of Endophytic Fungi from Polygala tenuifolia Willd

从10月份采集的栽培和野生远志(Polygala tenuifolia Willd)的根、茎、叶中分离内生真菌85株,其中自栽培远志分离33株,野生远志分离52株,共鉴定76株,隶属于23个属。通过对14种指示菌进行生长抑制试验,发现远志内生真菌对枯草芽孢杆菌、宋内氏痢疾杆菌、大肠杆菌、白色念珠菌和九州镰孢霉等5种指示菌抑制效果较好。经鉴定,它们属于镰孢霉属,交链孢霉属,Aphanocladium等属,对单核细胞增生李斯特菌、蜡样芽孢杆菌、普通变形杆菌、致病性大肠埃希氏菌、小孢拟盘多毛孢和深红酵母等6种指示菌抑制效果较差,对金黄色葡萄球菌、肠炎沙门氏菌和副溶血型弧菌没有抑制作用。

Eighty-five endophytic fungal strains were isolated from the roots, stems and leaves of Polygala tenuifolia Willd, among which, fifty-two from natural plants and thirty-three from cultivated ones. Seventy-six strains were classified as twenty-three fungal genera. The inhibitory activity screening to fourteen microbe were conducted research. The results showed that some endophytic fungi had remarkble inhibitory activities to Bacillus subtilis, Shigella sonnei, Escherichia coli, Candida albicans, Fusarium kyrushuense and they were all belonged to Fusarium, Alternaria, Aphanocladium respectively. All of the endophytic fungi isolated from Polygala tenuifolia showed no inhibitory activities to Staphyloccocus aureus, Salmonellae enteritis, Bibrio parahemolyticus.