摘要
对206个收集自世界各地的双孢蘑菇栽培菌株的总DNA进行大规模的SRAP、ISSR和RAPD分析,共获得15条SRAP、3条ISSR和2条RAPD特异性标记条带。利用这20个标记条带对206个菌株进行统计和聚类分析,获得亲缘关系树状图。结果显示在28%的相似值上,206个菌株可以分为高产型和优质型两大类群。其中,优质类群主要包含优质传统菌株(代表菌株8213、8211)和优质杂交菌株(代表菌株为福建省蘑菇菌种研究推广站选育的As2796、As4607)两大类,高产类群也主要包含高产传统菌株(代表菌株01、02)和高产杂交菌株(代表菌株为荷兰选育的U1、U3)两大类。而在100%相似值上,可分为大小72个类群,每群包含菌株数为1～31不等。
206 cultivation strains of Agaricus bisporus collected from all over the world were studied by usingt he techniques of SRAP,ISSR and RAPD fingerprinting,and 15 special SRAP bands,3 special ISSR bandsa nd 2 special RAPD bands were obtained.The strains were analyzed and clustered based on the 20 specialb ands and a dendrogram was obtained.The results showed that 206 strains be divided into high-productiong roup and good-quality group at the similarity coefficient of 28%.The good-quality group mainly containsg ood-quality traditional strains（with representative strain of 8213 and 8211）and good-quality hybrid strains（with representative strain of As2796 and As4607 bred by Fujian Mushroom R＆D Station）.Theh igh-production group mainly contains high-production traditional strains（with representative strain of 01 and0 2）and high-production hybrid strains（with representative strain of U1 and U3 bred by the Netherland）.Anda t the similarity coefficient of 100%,the strains were divided into 72 groups with strain number range of 1-31.
出处
《中国农学通报》
CSCD
北大核心
2009年第4期149-156,共8页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金项目"中国野生双孢蘑菇种质资源调查
鉴定与重要性状评价"(30671456)
福建省科技厅重大项目"福建省食用菌种质资源科技共享平台建设及相关技术研究"(2006S1001)
福建省自然科学基金重点项目"中国野生双孢蘑菇种质资源调查
鉴定与重要性状评价"(B0620004)
福建省农科院创新基金项目"利用野生种质创新选育双孢蘑菇抗逆新菌株"。
