柴胡根腐病致病菌生物学特性研究初报

Preliminary Study on Biological Characteristics of Root Rot Pathogen of Bupleurum chinense DC.

根腐病是目前对柴胡为害最为严重的病害之一,此文对该病病原菌的生物学特性进行了研究。结果表明,致病菌生长最适温度为25～30℃,最适pH6～8,在麦汁培养基上较在PDA培养基上容易产孢。孢子萌发的最适条件是20～25℃,pH4～6和100%相对湿度。孢子在25℃水滴中3h后开始萌发,10h时全部萌发,孢子临界致死条件为51℃,10min。

Root rot disease is one of the most destructive diseases of Bupleurum chinense DC.,biologicalc haracteristics were studied in this work.Results showed that the optimal temperature and pH for the grwth ofp athogen isolated were 25-30℃and pH 6-8.Also,we found that,which was easier to sporulate on oatmealm edium than PDA medium.The optimal condition for spores germination was 20-25℃,pH 4-6 and relativeh umidity 100%.Spores of pathogen begin to germinate in water drop 3 hours later,and germinated thoroughlya fter 10 hours in it,the critical lethal condition of spores was 51℃for 10 minutes.