摘要
根据GenBank上发表的鸡传染性支气管炎病毒(IBV)基因序列,针对IBV M基因全序列设计合成了一对引物,以IBV疫苗株为模板,建立了检测IBV的RT-PCR方法。应用该方法对IBV疫苗株RNA进行扩增,获得与预期大小相符,长度为1105 bp的特异性目的片段;敏感性测定该RT-PCR可扩增到18pg的IBV-M-cDNA。结果表明建立的RT-PCR方法对IBV的检测敏感性高、特异性强,可用于IBV感染的诊断和流行病学调查。
A pair of primers were designed and synthesized based on the sequences of the IBV genomes, and the RT-PCR method to detect the infectious Bronchitis virus (IBV)were developed.The 1105 bp specifical fragment were obtained from the IBV vaccine strain RNA by this RT-PCR.The sensitivity of RT-PCR reached to 2.85 ng IBV-M-cDNA. These results showed that the RT-PCR method to detect IBV were of better specificity and higher sensibility. The results showed that this PCR technique was specific and applied to IBV diagnoses and epidemiology investigation.
出处
《中国农学通报》
CSCD
北大核心
2009年第5期14-17,共4页
Chinese Agricultural Science Bulletin
基金
省财政专项-福建省农业科学院科技创新团队建设基金资助(编号:STIF-Y02)