发酵型金耳多糖的分离纯化及其降血糖活性研究

Isolation and Purification of Polysaccharides from Fermented Products of Tremella aurantialba and Hypoglycemic Effect

研究了发酵型金耳多糖(FTAP)的分离纯化方法及其降血糖生物学活性;比较了沸水煮提法和碱水热提法的差异,确定FTAP的提取工艺。Sevage法除蛋白,利用sephadex G-75凝胶柱层析进行分离纯化。采用四氧嘧啶诱导的高血糖大鼠模型,灌胃给药,监测血糖,测定脂代谢指标,对FTAP进行降血糖活性评价。结果得到:沸水煮提法最大多糖得率为15.5%,碱水热提法最大多糖得率为14.9%,选择沸水煮提法为制备FTAP的方法。利用sephadex G-75凝胶柱层析得到单一对称洗脱主峰,表明得到的FTAP为均一多糖。连续口服FTAP 7天时,44 mg/(kg天)和264 mg/(kg天)剂量组小鼠与模型组空腹血糖比较降血糖作用明显,血清甘油三酯水平也显著下降。证明FTAP可降低高血糖模型小鼠的高血糖水平。

The purpose of this paper is to isolate and purify the polysaccharides from fermented products of Tremella aurantialba （FTAP） and to study its hypoglycemic activities. The process of boiling-water extracting polysaccharides was investigated in comparison with that of alkaline solution extracting polysaccharides to decide the extraction protocol. Sevage method was used to precipitate the protein from the crude polysaccharides solution. The polysaccharides were separated and purified by Sephadex G-75 column chromatography. The hypoglycemic effect of FTAP was investigated in aUoxan-induced diabetic mice. The extraction rate of the polysaccharides by boiling-water process was 15.5%, and the extraction rate of the polysaccharides by alkaline solution process was 14.9% .The method of boiling-water extraction polysaccharides was selected to extract FTAP. The results of Sephadex G-75 column chromatography showed purified FTAP were gained. After orally administrated FTAP 44mg/（kgd） and 264mg/（kgd） to hyperglycemic mice for 7d, the glucose and TG in serum were decreased obviously, but serum TC did not change remarkably, compared with the control group. FFAP exhibited significant hypoglycemic activity in alloxan-induced diabetic mice.