HPLC法测定番泻叶滴丸中番泻苷B的含量

Determination of sennoside B in dropping pill of folium sannae by HPLC

建立番泻叶滴丸中番泻苷B的含量测定方法．采用高效液相色谱法测定含量；ODS柱，流动相A为乙腈，B为醋酸-醋酸钠缓冲溶液（pn值为5．0）（1：10），加入3．77g四庚基溴化氨，A与B的比例为14：86（V：V），检测波长为340nm，流速为1．0mL／min．番泻苷B的线性范围为0．4～1．4μg（r=0．9998），回收率（n=6）为98．33％（RSD=0．6％）．本方法简便准确，重现性好．

To establish a method for determining sennoside B in dropping pill of Folium Sannae. The determination is performed by HPLC using ODS column, mobile phase is a mixture of diluted pH value is 5.0 acetic acid-sodium acetate buffer solution（ 1： 10） and aeetonitrile （86： 14, V： V） containing 7.7 mmol/L tetra - n - heptylammonium bromide. Detection wavelength is 340 nm and a flow rate of 1.0 mL/min. The linearity range is 0.4 - 1.4 μg （ r =0. 999 8）. The average recovery is 98.33% （n =6） , RSD =0.6%. The method is simple, accurate and highly reproducible.