固定化乳酸菌发酵制备L-乳酸的初步研究

Production of L-lactic acid fermentated by immobilized lactobacillus plantarum

对影响固定化乳酸菌发酵玉米淀粉糖化液的主要因素进行了研究，并用响应面分析法优化了固定化发酵的培养基成分．结果表明，海藻酸钠质量分数为2％，凝胶颗粒直径在1．3～1．7mm为固定化乳酸菌的最优条件；糖化液140g／L，玉米浆76．3g/L，CaCO3 70g/L为固定化发酵的最佳培养基组成，在此条件下乳酸产量可达132．4g／L．

The main factors influence corn starch saccharification fermentated by immobilized lacto- bacillus plantarum is studied and optimization of culture medium for fermentation is carried out by response surface analysis methodology（RSM）. The results indicate that the optimum immobilization conditions acre as follows ： sodium algite 2%, immobilized beads diameter 1.3 - 1.7 mm; while the optimum composition of culture medium are： saccharification 140 g/L, corn steep liquor 76. 3 g/L, calcium carbonate 70 g/L,lactic acid content reachs 132.4 g/L