脑白质损害新生大鼠脑组织ephrin-B3表达及意义

Expression of ephrin-B3 mRNA in the brain of neonatal rats with white matter damage

目的建立新生大鼠脑白质损害(white matter damage,WMD)模型,探讨WMD新生大鼠脑组织ephrin-B3 mRNA的表达变化及意义。方法采用2日龄SD大鼠,通过结扎右侧颈总动脉并吸入6%氧气4h建立WMD模型(WMD组),分别于模型后0、8、24、48、72h及7d处死,同时设立相应假手术对照组。脑组织HE染色检测病理变化,荧光定量RT-PCR检测ephrin-B3mR-NA表达。结果WMD组缺氧缺血8h可见纹状体内细胞胞体肿胀、细胞稀疏、间隙增宽,7d可见缺氧缺血侧侧脑室旁白质呈筛网状坏死。与假手术组比较,WMD组脑白质ephrin-B3 mRNA在缺氧缺血后8、24、48、72h及7d表达均增加,差异有统计学意义(P<0.05)。结论新生大鼠WMD时,脑白质ephrin-B3mRNA表达显著增加,提示ephrin-B3参与了WMD过程中神经细胞的损伤及再生抑制机制。

Objective To study the expression of ephrin-B3 mRNA in the brain of neonatal rats with white matter damage （WMD）. Methods WMD models of 2 day-old SD rats were established by ligating the fight common carotid artery followed by exposure to 6% oxygen for 4 hours. Meanwhile, the sham controls were set up without ligation of the artery and hypoxia treatment. The rats were sacrificed and the brains were collected at 0, 8, 24, 48, 72 h and 7 d after bypoxic-ischemia. The pathologic changes of brain tissues were then observed by light microscope. Real time RT-PCR was applied to detect the expressions of ephrin-B3 mRNA. Results Tissue edema and cell death of brain were found in the WMD rats. The expressions of ephrin-B3mRNA were significantly increased at 8, 24, 48, 72 h and 7 d in the experimental groups compared with the control groups （P 〈 0.05 ）. Conclusion The expression of ephrin-B3 mRNA was significantly increased with WMD injury compared with the control groups, which suggests that ephrin-B3 may participate in the pathogenesis of WMD in neonatal rats.