Myogenin mRNA丰度在金华猪和长白猪背最长肌中的表达

Myogenin mRNA abundance in longissimus dorsi muscle of Jinhua pig and Landrace pig

运用半定量RT-PCR方法对不同生长阶段金华猪和长白猪背最长肌中肌细胞生成素(Myogenin,MyoG)基因mRNA的表达丰度进行测定,分析与肌肉沉积的关系。结果表明:在2种猪35、80、125日龄均可检测到MyoG mRNA,金华猪背最长肌中MyoG基因的表达随着年龄和胴体瘦肉率的增加而增加,并且MyoG基因表达与胴体瘦肉率呈正比;而长白猪背最长肌中MyoG基因的表达恰好相反。除35日龄以外品种间无显著差异。

Myogenin （Myogenin,MyoG） mRNA abundance in Longissimus dorsi muscle （LDM） of Jinhua pigs and Landrace pigs （35,80,125 days of age） were determined by semi-quantitative RT-PCR. The results showed that：during the whole expreimental period,the expression of MyoG gene increased with the age and muscle percentage of carcass in LDM of jinhua pigs,the expression levels of MyoG gene and muscle percentage of carcass were directly correlated. In contrast, the expression of MyoG gene decreased with the age and muscle percentage of carcass in Labdrace pigs,the expression levels of MyoG gene and muscle percentage of carcass were negatively correlated, but did not display breed differences（other than the 35 day-old outside）.