木薯花药愈伤组织诱导初步研究

Callus induction of anther in cassava(Manihot esculenta L.)

为探讨不同激素、低温对木薯花药愈伤组织诱导和分化的效果,以木薯华南5号的花药为外植体,接种于含不同激素组合的MS固体培养基,分别进行愈伤组织诱导、继代培养和分化。结果表明,在MS培养基中,随着2,4-D浓度的增加,木薯花药愈伤组织的诱导率不断升高,但诱导时间延长;在培养基中添加6-BA 3.0mg/L+NAA 0.2mg/L,木薯花药愈伤组织诱导率最高,达100%;在0～5d内,低温处理的时间越长,木薯花药愈伤组织诱导率越低,以不进行低温处理的诱导率最高;在分别含6-BA 3.0+NAA 0.2的MS培养基中,花药愈伤组织继续增殖,但未分化出不定芽和不定根,而在添加NAA 0.2mg/L+KT 0.5mg/L或2,4-D 0.5mg/L+KT 0.5mg/L的培养基中,愈伤组织可分化形成2～5条不定根。今后需在材料基因型、绿苗分化培养基、培养条件等方面对木薯花药培养诱导单倍体做进一步的深入研究。

In order to better understand the effects of different hormones, low temperature on the callus induction and differentiation of anther, the anthers of cassava variety SCA 5 were used as explants and inoculated to MS solid medium with different hormone combinations for the callus induction, subculture and differentiation of anther. The results showed that the induction rate of callus for anther of cassava was increasing with the increase of 2,4-D concentration in MS medium, but the induction stage was longer. The induction rate of callus for anther of cassava reached the highest for 100% in the medium adding 6-BA 3.0mg/L＋NAA 0.2mg/IL In 0-5 days, the longer treating time of low temperature, the lower callus induction rate of anther for cassava, and the induction rate of callus without low temperature treatment was the highest. Moreover, in the MS medium adding 6-BA 3.0mg/L ＋ NAA 0.2mg/L, the callus of anther was propagated continuously, but could not differentiate to produce bud and root, while the callus could produce 2-5 adventitious roots in MS adding NAA 0.2mg/L ＋ KT 0.5mg/L or 2,4-D 0.5mg/L ＋KT 0.5mg/L In the future, the haploid induction of anther for cassava could he carried out from the genotype of material, differentiation culture medium and conditions of plantlet culture, and so on.