穿心莲内酯对小鼠呼吸道免疫功能的影响

Effects of andrographolide on respiratory tract immune functions of mice

通过给肺炎模型小鼠腹腔分别注射高剂量(200 mg.kg-1.d-1)和低剂量(100 mg.kg-1.d-1)的穿心莲内酯,7 d后,测定小鼠免疫器官和肺泡灌洗液(BALF)中相关免疫指标的变化,探讨穿心莲内酯对呼吸道免疫功能的影响。结果表明,穿心莲内酯高剂量组小鼠的脾和胸腺指数、支气管肺泡灌洗液(BALF)中分泌型免疫球蛋白A(sIgA)和干扰素-γ(IFN-γ)的含量均显著低于生理盐水组(p<0.01);而BALF中巨噬细胞吞噬率则显著升高(p<0.01)。说明穿心莲内酯能增强巨噬细胞吞噬功能;并对脾、胸腺等免疫器官的生长,支气管sIgA分泌及IFN-γ表达有显著的降低,起到减缓或消退肺部炎症的作用。

To investigate the effects of andrographolide on the respiratory tract immune functions of mice, the pneumonia model mice were treated with different dosages of andrographolide （ 100 mg.kg^-1. d^-1 and 200 mg.kg^-1. d^-1 ） by peritoneal injection. Seven days later, the immunity relation indexes of pneumonia model mice were tested. The result showed that compared with the normal saline group, the thymus index, the spleen index, the contents of secretory immunoglobulin A and interferon -γ in the mice BALF of high andrographolide dosage group decreased significantly （p 〈 0.01 ）. The phagotrephic rate of macrophages in BALF of high andrographohde dosage group increased significantly （p 〈 0.01 ）. It indicated that the macrophages phagocytosis of pneumonia model mice enhanced and the growth of immunity organs, the secretion of slg A and the IFN -γ were significantly inhibited by andrographolide.