生防菌株XF-1的鉴定和抑菌谱的测定

Identification and Fungistatic Effect of a Biocontrol Strain,XF-1

依据传统形态学、生理生化特征及16S rRNA基因序列,将一株对十字花科根肿病有很好防治效果的菌株XF-1鉴定为枯草芽孢杆菌。采用细菌的通用引物P0,P6扩增16S rRNA基因片段,得到1513 bp的DNA片段,其序列与枯草芽孢杆菌的序列的同源性高达99%。与病原真菌对峙培养的结果表明,该菌株能对供试的卵菌、子囊菌、担子菌和半知菌的21个植物病原真菌均有很好的抑制作用。

A biocontrol strain, XF-1, was identified as Bacillus subtilis based on morphology, physiology, biochemistry and biotechnology, which could control clubroot of cruciferous crops effectively. The sequence of 16S rRNA gene has 99% identity to B. subtilis according to the amplified fragment of 1513 bp with a pair of universal primers, P0 and P6, of bacteria. On co-culture plates this strain showed high suppression effect on 21 fungal pathogens involved in oomycetes, ascomycetes, basidiomycetes and deuteromycetes.