半夏无糖组培苗营养生长和光合生理对增施CO_2的响应

Response of Nutritional Growth and Photosynthesis to Enriching CO_2 in Pinellia ternata Plantlet of Sugar-free Tissue Culture

以半夏叶片通过组织培养产生的不定芽为材料，探讨在微环境调控条件下，半夏无糖组培苗营养生长和光合生理对增施CO2的响应。研究结果表明：在CO2浓度600～1800μmol／mol的范围内，随CO2浓度的增加，半夏无糖组培苗的叶面积、生根率、净光合速率、水分利用率、叶绿素含量逐渐升高，气孔导度、蒸腾速率则逐渐下降。当CO2浓度为（1500±50）μmol／mol时，半夏无糖组培苗叶片的净光合速率达最大值（10．20±0．27）μmol／（m^2·s），且组培苗长出大量须根。研究表明，（1500±50）μmol／mol是半夏无糖组培苗进行光合作用的最适CO2浓度。

In this paper, adventitious buds of Pinellia ternata were used as material and the response of nutritional growth and photosynthesis to enriching CO2 in P. ternata plantlet of sugar-free tissue culture was discussed. The results showed in the CO2 concentration range of （600 - 1 800） μmol/mol, with the increasing of CO2 concentration, the leaf area, rooting rate, net photosynthetic rate （ Pn）, Water use efficiency （WUE） and chlorophyll content of P. ternata plantlet of sugar-free tissue culture were raised gradually, but the transpiration rate （Tr） and stomatal conductance （Gs） declined. While the CO2 concentration reached （ 1 500 ± 5 ） μmol/mol, the Pn reached the highest value of （ 10. 20 ± 0. 27） μmol/ （m^2 · s） and lots of fibers grew. So （ 1 500 ±50）μmol/mol was the optimal CO2 concentration for the photosynthesis of P. ternata plantlet of sugar-free tissue culture.