滴水观音的快繁技术研究

Research on Tissue Culture and Rapid Propagation of Alocasia macrorrhiza

采用滴水观音休眠芽为外植体，进行组培快繁技术体系研究。结果表明：诱导愈伤组织的最佳培养基配方为MS＋6-BA1．0mg／L＋NAA0．1mg／L，且上表面切除0．1～0．3cm有利于愈伤组织形成；最佳不定芽增殖培养基为MS＋6-BA2．0mg／L＋NAA0．1mg／L＋香蕉泥80g／L；且下表面切除0．1—0．2cm有利于愈伤组织增殖与分化；最佳生根培养基1／2MS＋NAA1．0mg／L＋6-BA0．01mg／L。

In this article, the rapid propagation technology system of Alocasia macrorrhiza tissue was studied with dormancy bud as material. The results showed that MS ＋ 6 - BA 1.0 mg/L ＋ NAA 0. 1 mg/L medium could induce variabile callus better than general methods, and curing 0. 1 -0. 3cm from up the surface of the material help variabilis callus proliferation and differentiation ; MS ＋ 6 - BA 2.0 mg/L ＋ NAA 0. 1 mg/L ＋ banana mash 80 g/L medium had the optimal effect on shoot reproduction ; 1/2MS ＋ NAA 1.0 mg/L ＋ 6 - BA 0. 01 mg/L medium had the optimal effect on roots, and cutting the bottom surface of the materials by 0. 1 -0. 2 cm was conducive to variabilis callus proliferation and differentiation.