摘要
采用滴水观音休眠芽为外植体,进行组培快繁技术体系研究。结果表明:诱导愈伤组织的最佳培养基配方为MS+6-BA1.0mg/L+NAA0.1mg/L,且上表面切除0.1~0.3cm有利于愈伤组织形成;最佳不定芽增殖培养基为MS+6-BA2.0mg/L+NAA0.1mg/L+香蕉泥80g/L;且下表面切除0.1—0.2cm有利于愈伤组织增殖与分化;最佳生根培养基1/2MS+NAA1.0mg/L+6-BA0.01mg/L。
In this article, the rapid propagation technology system of Alocasia macrorrhiza tissue was studied with dormancy bud as material. The results showed that MS + 6 - BA 1.0 mg/L + NAA 0. 1 mg/L medium could induce variabile callus better than general methods, and curing 0. 1 -0. 3cm from up the surface of the material help variabilis callus proliferation and differentiation ; MS + 6 - BA 2.0 mg/L + NAA 0. 1 mg/L + banana mash 80 g/L medium had the optimal effect on shoot reproduction ; 1/2MS + NAA 1.0 mg/L + 6 - BA 0. 01 mg/L medium had the optimal effect on roots, and cutting the bottom surface of the materials by 0. 1 -0. 2 cm was conducive to variabilis callus proliferation and differentiation.
出处
《云南农业大学学报(自然科学版)》
CAS
CSCD
北大核心
2009年第2期312-315,共4页
Journal of Yunnan Agricultural University:Natural Science
基金
教育部春晖计划资助项目(Z2005-2-65008)
关键词
滴水观音
休眠芽
组培快繁
Alocasia macrorrhiza
dormancy bud
tissue culture and rapid propagation