恩诺沙星单克隆抗体的制备及酶联免疫分析方法的建立

Production of Monoclonal Antibodies Against Enrofloxacin and the Establishment of Enzyme-linked Immunosorbent Assay

采用抗恩诺沙星单克隆抗体建立酶联免疫吸附法(ELISA)用以检测恩诺沙星在动物源性食品中的残留。经方法学鉴定,本方法的检测范围为0.5～50μg/L,灵敏度为0.2μg/L,批内变异系数<10%,批间变异系数<20%。鸡肉、鱼肉、虾和蜂蜜样品的回收率分别为95.5%～107.5%、80.0%～101.3%、105.7%～122.7%和93.3%～111.3%。方法学鉴定结果符合免疫分析的基本要求。

An enzyme-linked immunosorbent assay （ELISA） using monoclonal antibodies against enrofloxacin （ENR） was developed to analyzed ENR residues in animal-based food products. The standard line of the assay was in the rang of 0.5-50μg/L. The sensitivity was 0.2μg/L. The intra- and inter-assay CVs of 3 samples were lower than 10% and 20% respectively. The recoveries of chicken muscle tissues, fish, shrimp and honey were in the range of 95. 5%-107. 5%,80. 0%-101. 3%, 105. 7%-122. 7% and 93. 3%-111.3% respectively.