^(188)Re直接标记抗体方法研究

Study of Direct Labeling Antibody With ^(188)Re

以2-巯基乙醇作为抗体的还原剂,葡萄糖酸钠作为中间弱配体,在pH5.0的条件下,以SnCl2快速还原188ReO4-,进行了单克隆抗体IgG的直接标记。探讨了影响标记率的因素。结果显示,优化后的标记条件为pH5.0,室温下标记3 h,SnCl2用量2.66μmol,葡萄糖酸钠用量80μmol。该方法操作步骤简单方便,标记率较高,为94.4%±0.7%,标记物不需分离纯化,且有良好的体外稳定性,室温下可稳定保存8 h。

A method of direct labeling IgG with 188 Re was studied. The labeling was conducted at pH5.0 with 2-ME and SnCl2 used as the reductant of antibodies and 188 ReO4^- respectively, and Sodium gluconate as chelate agent. The effecting factors on the labeling yield were evaluated. The labeling procedure was easy and convenient to perform, and could obtain high labeling yield （94.4%±0.7%） without further purification. The labeled antibodies were stable in vitro. Therefore the direct labeling approach has more practical application prospects.