高效液相色谱与电子顺磁共振谱联用技术测定自旋标记抗癌药物

Separation and determination of spin-labled anti-tumor podophyllotox in derivatives by HPLC coupled with EPR

利用高效液相色谱（HPLC）与电子顺磁共振谱（EPR）联用技术，进行了高效液相色谱分离自旋标记抗癌药物的条件实验，考察了流动相比例，最低检出量等色谱行为，得到3种自旋标记抗癌药物的满意分离及对动物小白鼠的药代谢动力学实验。讨论了液相色谱流出液引进电子顺磁共振进行定量测定的研究方法。联用技术用于自旋标记抗癌药物分析，方法简便，快速，准确，实际应用的结果甚为满意。

A method for separation and determination of spin-labeled anti-tumor podophyllotox in derivatives by HPLC coupled with electron paramagnetic resonance （EPR） has been developed. The analytical conditions of reverse phase HPLC have been discussed and an separation of GP-3, GP-5, GP-7 was obtained by using MeOH-H2O system. The detection limits were 10^-8g/mL. EPR detector was then used to determine HPLC. This new technique has been proved to be useful for pharmacokinetics study of spin-labeled anti-tumor podophyllotoxin derivatives. This method was assayed. It is simple, rapid and accurate.