生物细胞与组织超弱发光探测系统研制

Development of detection system for ultraweak photon emission from biological cells and tissues

为了实现对生物超弱发光现象的研究,研制该探测系统,以用于在常温下对生物样本的超弱发光现象进行测量。采用多种措施降低外界环境对探测系统的影响:选择灵敏度高、暗电流低的光电倍增管作为核心探测器件;制作测量系统专用暗箱保证测量环境的高信噪比和稳定性;设计了进行生物细胞与组织超弱发光实验的多波长激励光源。经过多次实验,系统在11.5℃环境温度下的本底噪声的平均值为43.5 CPS,取得了较好的效果。系统信噪比高,稳定性好,能够在常温下对生物的超弱发光和延迟发光现象进行观察,达到了预期目的。

The development of detection systems for the study of ultraweak photon emission at room temperature is discussed. In order to reduce noise, many kinds of meathods are adopted. The high-sensitivity and low darkcurrent PMT is used to be the key detector. The measurement box has high SNR （signal-to-noise） and measurement environment stability. The multi-wavelengh stimulating light source is designed for extra-weak bioluminescence from living cells and tissues. The mean of background noise of the system is 43.5 CPS at room temperature 11.5℃. The system has high SNR and good stability. It can be used for studying ultraweak cell and tissue radiation at room temperature.