人参二醇组皂苷增强顺铂对人前列腺癌DU145细胞凋亡的效应

Panoxadiol saponin enhances the apoptotic effect of cisplatin on prostatic cancer cells DU145

目的探讨人参二醇组皂苷(panoxadiol saponin PDS)与顺铂(cisplatin,DDP)联合应用对DU145前列腺癌细胞增殖及凋亡的影响。方法采用MTT比色法检测PDS与DDP联合应用对DU145细胞增殖活力的影响;吖啶橙染色观察诱导细胞凋亡情况;流式细胞仪分析细胞周期及凋亡,并计算细胞平均凋亡率;免疫化学和Western blot技术观察细胞内激活的caspase3的表达。结果100mg/L PDS与0.2μmol/L DDP联合给药:(1)48h后可使单独应用0.2μmol/L DDP组其肿瘤细胞生长抑制率由16.35%提高到47.13%;(2)吖啶橙荧光染色可见细胞呈现明显凋亡形态,其凋亡率与2μmol/L DDP组相当;(3)流式细胞术结果显示,可使单独应用0.2μmol/L DDP组其诱导DU145细胞凋亡率从5.53%提高到19.39%,相当于其10倍剂量即2.0μmol/L DDP的诱导凋亡效应(21.05%),明显高于PDS单独应用的凋亡率;(4)免疫化学和Western blot结果显示,可使单独应用0.2μmol/L DDP组细胞内激活的caspase3阳性细胞率与2μmol/L DDP组相当。结论PDS能增强DDP对DU145前列腺癌细胞的致凋亡效应。

Objective To investigate the effect of cisplatin（DDP） combined with panoxadiol saponin （PDS） on proliferation and apoptosis of prostatic cancer cells DU145 in vitro. Methods The proliferation of treated cells with PDS and DDP were assessed by MTT test. Apoptotic cells were analyzed by acridine orange staining using Flow Cytometry. The expression of caspase3 in cells were detected by immunochemistry and Western blot. Results DU145 cells were treatedwith 100mg/L PDS and/or 0.2 umol/LDDP： （1）the inhibition rate of cell growth increased from 16.35% to 47.13% in 0.2umol/L DDP group 48 hours after treatment; （2）Mophology of apoptotic cells cound be found by acridine orange staining in PDS and DDP group, the apoptotic rate was silimar with that of 2 umol/L DDP group; （3）the apoptotic rate of cells were elevated from5.53% to 19.39%, which was similar effect with 2.0 umol/LDDP（21.05%）;（4）The expression of active caspase3 in 0.2 umol/LDDP and 100mg/L PDS group was found to be upregulated which was similar with that of 2.0 umol/LDDP. Conclusion PDS might enhance the apoptotic effect of DDP on prostatic cancer cells DU145.