男性非淋菌性尿道炎患者尿道黏膜细胞内广泛存在衣原体始体与包涵体

Abroad existence of Chlamydial initial and inclusion bodies in urethra mucous membrane epithelia cells of male NGU patients

目的探索男性非淋菌性尿道炎患者尿道黏膜细胞内衣原体始体与包涵体检测的意义。方法取患者尿道粘膜柱状上皮细胞涂片姬姆萨染色镜检。结果男性非淋菌性尿道炎患者尿道黏膜细胞内广泛存在衣原体始体与包涵体,在衣原体敏感抗生素治疗前后衣原体感染细胞百分比差异有统计学意义(P<0.01),尿道细胞标本经实时荧光定量聚合酶链反应检测含有衣原体DNA,经免疫组化染色检测含有衣原体抗原抗体免疫复合物。结论提示衣原体是男性非淋菌性尿道炎患者的主要致病菌,尿道黏膜细胞内存在衣原体包涵体与数个始体是衣原体感染的确凿证据。

Objective To Ddetect the infection status ofing Chlamydial initial and inclusion bodies in urethra mucous membrane epithelia cells of male nongonococcus urethritis （NGU） patients. Methods Epithelia cells of urethra mucous membrane derived from three hundred and nineteen patients with NGU were stained with Giemsa for morpho- logical examination. Results The Chlamydial initial and inclusion bodies were discovered in epithelia cells of urethra mucous membrane by Giemsa staining. There was a significant difference in the percentage of Chlamydial-infected urethra cells between before and after the treatment with antibiotics （P〈0.01）. Chlamydial DNA was detected in urethra cells by RT-PCR, and its antigen-antibody complex was also examined with immunohistochemistry analysis. Conclusion The Chlamydial initial and inclusion bodies detected in urethra mucous membrane epithelia cells indicated that there existed chlamydial infection in NGU patients and Chlamydial was the main pathogen of NGU patients.