微卫星标记分析乌江流域白甲鱼群体的遗传多样性

A Preliminary Analysis of Genetic Diversity of Population of Onychostoma sima in Wujiang River with Microsatellite Markers

以乌江流域白甲鱼(Onychostoma sima)为材料,构建了白甲鱼微卫星富集文库。对乌江彭水电站坝上、坝下种群进行PCR扩增,得到5个多态位点,其等位基因数目3～6;多态信息含量0.4465～0.7116;观测杂合度0.4444～0.9310;期望杂合度0.4902～0.7624;Hardy-Weinberg平衡偏离指数-0.4171～0.2380,各位点两两之间不存在显著的连锁不平衡现象。实验初步表明:乌江流域白甲鱼的遗传多样性较为丰富,但彭水水电站的建立已经对其造成了一定程度的影响。

The microsatellite markers of Onychostoma sima were isolated and the genetic diversity were analysed in this study. A library of partial small size fractionated genomic DNA was constructed with the O. sima in Wujiang river. Five polymorphic microsatellite loci were obtained. Genetic variation in a sample of 32 individuals was quantified by the number of alleles, which ranged from three to six, and PIC changed from 0. 4465 to 0.7116, and by the observed and expected heterozygosities ranging from 0. 4444 to 0. 9310 and from 0. 4902 to 0. 7624 per locus, respectively, and by Hardy-Weinberg departure value （D） ranging from - 0. 4171 to 0. 2380. Linkage equilibrium was not observed. The findings indicated that although the genetic diversity of O. sima in Wujiang river was rich, it had been influenced by the construction of Pengshui hydropower station on the Wujiang river to a certain extent.