噬菌体生物扩增法检测结核性胸膜炎患者胸水临床应用研究

The clinical value of pleural fluid detection for the diagnosis of tuberculous pleurisy by phage am-plified biologically assay

目的评价噬菌体生物扩增法检测胸水中结核分枝杆菌对诊断结核性胸膜炎的临床应用价值。方法采用噬菌体生物扩增法技术对73例结核性胸膜炎患者,20例非结核性胸膜炎患者胸水中的结核分枝杆菌进行检测,同份标本同时进行涂片抗酸染色、罗氏培养。结果73例结核性胸膜炎胸水,噬菌体生物扩增法阳性率为49.3%(36/73)、涂片抗酸染色阳性率为2.7%(2/73)、罗氏培养阳性率为4.1%(3/73)。20例非结核性胸膜炎,噬菌体生物扩增法、涂片抗酸染色、罗氏培养都未检出阳性。结论噬菌体生物扩增法检测胸水中结核分枝杆菌只需2天时间,操作简便、灵敏度高、特异性强,不需要特殊仪器设备,可作为诊断结核性胸膜炎的一种好方法。

Objective To evaluate the clinical value of phage amplified biologically（PhaB） assay in pleural fluid detection for the diagnosis of tuberculous pleurisy. Methods The pleural fluid samples, from 73 patients with tuberculous pleurisy and.20 patients with non-tuberculous pleurisy, were detected by PhaB assay. Results The positive rate of PhaB assay in 73 patients with tuherculous pleurisy was 49.3%（36/73）, those of smear and culture were 2.7%（2/73） and 4.1%（3/73） respectively. 20 samples with non-tuberculosis pleurisy were all negative by above three methods. ConcluSions The experimental procedure used PhaB assay costs only 2 days, and it is simple, highly sensitive and specificity method for the diagnosis of tuberculous pleurisy.