低强度He-Ne激光照射对离体小鼠巨噬细胞Ca^(2+)浓度的影响

Effect of Low Power HeliumNeon Laser Irradiation in vitro on Intracellular Calcium Concentration in Mouse Macrophages

为研究低强度Ｈｅ－Ｎｅ激光照射对离体小鼠巨噬细胞内Ｃａ２＋浓度的影响以及其与能量密度（剂量）的关系，用Ｆｌｕｏ－３乙酰甲脂对巨噬细胞Ｃａ２＋作荧光标记，应用图象分析系统检测激光照射后细胞内Ｃａ２＋的荧光强度变化。结果：Ｈｅ－Ｎｅ激光照射引起细胞内Ｃａ２＋浓度改变。照射５ｍｉｎ，激光剂量为０．６７９Ｊ／ｃｍ２，细胞荧光强度出现非常明显的增强；照射１０ｍｉｎ，剂量为１．３５８Ｊ／ｃｍ２，细胞荧光强度增强达到最大值；照射１５ｍｉｎ，剂量为２．０３７Ｊ／ｃｍ２，细胞荧光强度减弱；照射２０ｍｉｎ，剂量为２．７１６Ｊ／ｃｍ２，细胞荧光强度降低至对照组之下。低强度Ｈｅ－Ｎｅ激光照射巨噬细胞可引起细胞内Ｃａ２＋浓度上升，产生细胞内信号传递，进而控制细胞功能。

he purpose of this study is to investigate the relation of HeNe laser irradiation and its energy intensity and the intracellular calcium concentration in mouse macrophages. The intracellular calcium was labeled with Fluo3 AM and the fluorescence intensity of the macrophages after laser irradiatiion in vitro was determined by a VIDAS image analysis system. Changes of the intracellular Ca2+ concentrations were revealed after HeNe laser irradiation. The cellular fluorescence intensity increased obviously after 5 minutes irradiation with a light dose of 0679 J/cm2. The maximum value of the cellular fluorescence intensity was observed after 10 minutes laser irradiation with a light dose of 1358 J/cm2. A decreasement of the cellular fluorescence intensity occurred at 15 minutes irradiation with a light dose of 2037 J/cm2, and the intracellular fluorescence intensity was lower than that of control group after 20 minutes laser irradiation with a light dose of 2716J/cm2. The rise of the intracellular Ca2+ concentration in macrophages induced by low power HeNe laser irradiation may affect the intracellular signal transmission, controlling the cellular function. Our findings may provide certain evidence in explanation of the mechanisms of clinical HeNe laser therapy.