摘要
HPLC测定触苯工人尿中苯基乙酰半胱氨酸的研究计时华龚立行朱心强何美景①马威②马新强②王莉③浙江医科大学卫生检测中心(杭州310006)苯基乙酰半胱氨酸是近年来发现的苯在人体内的代谢产物之一。本文用高效液相色谱仪,紫外检测器(256nm)、RP—O...
S-phenyl-N-acetylcysteine(PhAc) is one of metabolite of benzene. The PhAc, in the urine of worker exposed to benzene, are analyzed by HPLC with a C 18 reversed-phase columu and methanol-water-phosphoric acid buffer and ulteaviolet detection at 256nm. The detection limits of PhAc was 2ug/ml. The linear range of determination was 0-30 ug/ml (r=0.998)with recovery above 96% and relative standard deviction of less than 10% The mothod of collect and preserve urine samples were investigated, and correlation between concentrations of urine PhAc and benzene in ari in workshop was probed.
出处
《中国卫生检验杂志》
CAS
1998年第2期74-77,共4页
Chinese Journal of Health Laboratory Technology
基金
浙江省卫生厅
浙江省教委
浙江省分析测试基金
关键词
HPLC
苯
PhAc
尿
enzene, S-phenyl-N-acetylcysteine, HPLC