牙科抗菌磷酸锌黏固剂的制备及其抗菌性能的检测

Preparation and determination of antibacterial activity of dental antibacterial zinc phosphate cement

目的探讨抗菌磷酸锌黏固剂的加工工艺及其抗菌性能。方法采用研磨法将载银无机抗菌剂添加在磷酸锌黏固剂粉体中合成载银抗菌磷酸锌黏固剂。以普通磷酸锌黏固剂作为对照,进行大体观察和扫描电镜观察。然后通过细菌(变异链球菌、白假丝酵母菌、牙龈卟啉单胞菌)接种于抗菌磷酸锌黏固剂上,计算出样品的抗细菌率。结果大体观察显示,抗菌磷酸锌黏固剂与普通磷酸锌黏固剂无明显差异。扫描电镜观察显示,抗菌磷酸锌黏固剂表面均匀散布Conval PAg-40颗粒。抗菌磷酸锌黏固剂对变异链球菌、白假丝酵母菌、牙龈卟啉单胞菌均有抗菌效果,且抗菌能力区别明显。结论添加载银抗菌剂对磷酸锌黏固剂的表面结构无明显影响且具有一定的抗菌性。

Objective To prepare and explore the antibacterial activity of dental antibacterial zinc phosphate cement （ZPC）. Methods Silver base inorganic antibacterial agent （Conval PAg-40） was mixed into ZPC powder with mortar. To verify the process technique, the structure of antibacterial ZPC was observed grossly and scanned by electronic microscopy, and was compared with the common ZPC. Then inoculate quantitative oral pathogens （Streptococcus mutants, Saccharomyces albicans, Porphyromonas gingivalis） on zinc phosphate cement. Viable count in the samples was detected after cultured for a certain time and the antibacterial rate was figured out. Results There was little difference between the antibacterial ZPC and the common ZPC. When observed by a scanning electronic microscope, Conval PAg-40 were well dispersed in ZPC and there were few air bubbles and crannies in the structure. Antibacterial ZPC had antibacterial effect to the pathogens and the antibacterial effects had significant difference. Conclusion There is little influence on surface structure of the ZPC. The ZPC which added silver based inorganic antibacterial agents has determinate antibacterial activity.