胃癌D17S261和D17S799位点二核苷酸重复序列不稳定性的意义

Clinical implication of dinucleotide repeat sequence instability in gastric cancer

目的研究二核苷酸重复序列不稳定性〔ＤＲＳＩ〕在胃癌发生中的作用及其临床意义．方法采用ＰＣＲ方法检测了Ｄ１７Ｓ２６１和Ｄ１７Ｓ７９９位点二核苷酸重复序列不稳定性．结果胃癌总ＤＲＳＩ发生率为３４％（１７／５０），其中高中分化腺癌ＤＲＳＩ阳性率（６６７％，１０／１５）显著高于低分化癌（１９４％，６／３１，Ｐ＜００１）；肠型胃癌ＤＲＳＩ阳性率（５５６％，１０／１８）显著高于胃型胃癌（２０％，６／３０，Ｐ＜００５），ＤＲＳＩ与胃癌部位、大小、浸润、分期、淋巴结转移无显著相关．结论ＤＲＳＩ在胃癌的发生中可能起重要作用．

AIM To investigate dinucleotide repeat sequence instability (DRSI) in the carcinogenesis of gastric cancer. METHODS DRSI was detected in 50 surgically resected specimens of gastric cancer using the PCR method. RESULTS DRSI was detected in 17 (34 0%) of the 50 gastric cancer specimens. The frequency of DRSI in well differentiated gastric cancer (66 7%, 10/15) was significantly higher than that in poorly differentiated one (19 4%,6/31) ( P <0 01), and in intestinal type (55 6%,10/18) it was significantly higher than in diffused type (20%,6/30) ( P <0 05). No significant correlation was found between DRSI and the size, location, lymphnode metastasis, serosal invasion and clinical stage of gastric cancer. CONCLUSION DRSI may be an early molecular marker and may play an important role in carcinogenesis of gastric cancer.