孕鼠维生素D缺乏对子鼠长骨维生素D受体蛋白表达的影响

Effects of vitamin D deficiency during pregnancy on femoral VDR protein expression of offspring

目的:研究大鼠孕期维生素D(VitD)缺乏对其子鼠长骨中维生素D受体(VDR)蛋白表达的影响。方法:成熟雌性SD大鼠36只随机分为模型组和对照组各18只,模型组以不含VitD的大鼠饲料避光喂养,对照组以标准饲料正常光照喂养。2周后与成熟SD雄性大鼠交配,分别取孕20天(E20d)、生后1天(B1d)和生后7天(B7d)子鼠左侧股骨。用Western blotting方法检测子鼠股骨VDR蛋白水平的表达。结果:VDR蛋白在E20d、B1d和B7d子鼠股骨的表达量,模型组均显著低于对照组(P均<0.05)。结论:孕鼠VitD缺乏可下调子鼠长骨VDR蛋白表达水平,可能为子鼠骨骼发育不良机制之一。

Objective：To study the expression of VDR protein in the femurs of offsprings whose mothers were deficient of vitamin D： Methods：Thirty-six adult female SD rats were chosen and divided randomly into the control group（ 18 rats） and model group（ 18 rats）. The control group was received vitamin D fodder with normal solarization and the model group received non-vitamin D fodder without solarization for 2 weeks. Then the female rats in two groups copulated with the male normal rats. The left femurs were collected from the 20-day embryos and the 1-day and 7-day newborns. VDR protein expression was measured by Western blotting. Results：The VDR protein expression of the model group was lower than the control group （P 〈 0.05）. Conclusion：The change of expression of VDR protein was due to vitamin D deficiency during the pregnancy, which may be the reason of offsprings＇ skeletal deformity.