TNF-α诱导Molt-4细胞周期特异性凋亡及cyclin B1的表达

TNF-α induces the specificity apoptosis of Molt-4 cells and the expression of cyclin B1

目的:观察TNF-α诱导Molt-4细胞的凋亡现象以及cyclinB1的表达,探讨膜受体途径细胞凋亡机制。方法:用TNF-α处理指数生长期的Molt-4细胞;应用API、免疫印迹电泳等方法检测细胞凋亡和cyclinB1的表达。结果:Molt-4细胞经TNF-α诱导后出现了明显的细胞凋亡,细胞凋亡主要发生在细胞周期的G1期,cyclinB1在G1期呈阳性表达。结论:TNF-α诱导的细胞凋亡具有细胞周期特异性,cyclinB1在G1期细胞中表达升高可能与G1期细胞凋亡有关。

bjective：To observe phenomenon of Moh-4 cell apoptosis induced by TNF-αand expression of cyclin B1, and investigate the receptor-mediated apoptosis mechanism. Methods：Exponentially growing human leukemia cell lines （Molt-4） were treated with TNF-α Method of API was used to detect the apoptosis, and method of western blot was applied to illustrate expression of cyclin B1. Results：Moh-4 cells which were treated by TNF-α went to apoptosis. Apoptosis was in G1-phase, and the expression of cyclin B1 was observed in G1-phase. Conclusion：Cell apoptosis induced by TNF-α was closely related to cell cycle and presented cell-cycle-specificity. The expression of cyclin B1 increased in G1-phase, which may be related to cell apoptosis in G1 .