食管癌患者T淋巴细胞核仁形成区嗜银蛋白表达与细胞周期的关系

Correlative analysis between peripheral T lymphocyte AgNORs and cell cycle from patients with esophageal carcinoma

目的探讨外周血T淋巴细胞间期核和分裂期染色体AgNORs的相关性及临床应用价值。方法应用银染色技术及KL型图像分析系统,检测74例食管癌和50例对照组的T淋巴细胞间期核和分裂期染色体AgNORs,结果分别以银染核仁面积与细胞核面积的百分比值(I.S%)和AgNORs数/细胞表示。结果食管癌组间期核AgNORs的I.S%(5.28±0.86)显著低于对照组(7.56±0.78)(P<0.001),分裂期染色体Ag-NORs数/细胞(5.64±0.45)也显著低于对照组(7.35±0.55)(P<0.01);食管癌组和对照组的这两种指标呈正相关,相关系数分别为r=0.82和r=0.87,均为P<0.01。结论外周血T淋巴细胞间期核AgNORs的I.S%和分裂期染色体AgNORs数/细胞均可作为肿瘤的辅助诊断指标,但前者检测方便、能自动计数,结果准确可靠,比后者更适合在临床推广应用。

[Objective] To investigate the AgNORs＇ correlation between peripheral T lymphocyte nucleus and chromosome. [Methods] Silver straining technique and KL image system were used to detect AgNORs of peripheral T lymphocyte nucleus and chromosome in 74 patients with esophageal carcinoma and 50 healthy controls. The results were expressed in the ratio of silver staining nucleolus area to nucleus area （I.S%）, and chromosome AgNORs counts （AgNORs counts/cell）. [Results] The I.S% of AgNORs and chromosome AgNORs counts in patients with esophageal carcinoma[（5.28±0.86）, （5.64±0.45）] were lower than healthy controls[（7.56±0.78）, （7.35±0.55）]（P 〈0.01）. In patients with esophageal careinomaand healthy controls, the I.S% of AgNORs was positively correlated with chro- mosome AgNORs counts. Correlation coefficient were 0.85 and 0.87, respectively （P 〈0.001）. [Conclusion] The I. S% of AgNORs and chromosome AgNORs counts in peripheral T lymphocytes can be served as two useful immunologic diagnostic makers, but the former presents a more convenient and credible approach for clinical application than the latter.