脑内胰岛细胞移植治疗糖尿病机理的初步研究

A preliminary study on the mechanism of treating diabetes mellitus by intracerebral transplantation of islet cell cluster(ICCs)

为研究非神经组织─—胰岛细胞脑内移植治疗糖尿病的可能及机理，分别对正常兔（n＝8）行ActrapidHM6U皮下及鞘内注射，以及阿托品0．5mg阻滞神经后再次鞘内注射的急性试验，并对10只四氧嘧啶高血糖兔行多次小剂量脑内注入ICCs的慢性研究。急性研究显示：中枢给胰岛素引起最强最长的低血糖及高胰岛素血症，同时伴随动物饮食、水量的减少，阿托品0．5mg可部分减轻此反应。而近2年的脑ICCs移植结果示，在（2～13）次×106个ICCs（含胰岛2～10×103）移植后，5／10糖尿病兔血糖缓慢下降（P＜0．05），3／5血糖已恢复正常，它们脑组织中IRI含量显著大于正常兔。提示Ins可能作为神经介质，通过自主神经纤维参与中枢对外周胰岛素分泌及血糖的调节。脑在长期超生理水平胰岛素刺激下，可能通过神经-内分泌网络放大胰岛素的外周效应，并可能对胰岛的再生、修复起了重要作用。

In order to investigate the effects of intracerebral transplantation of islet cell clusters (ICCs) on diabetes acute study was done with & normal rabbils given separately Actrapid HM 6U subcutaneously and intrathecally and Atropine 0. 5mg before Actrapid 6U intrathecally. As a chronic study alloxan DM rabbits(n = 10) were intracerebrally autografted by small doses of 106 ICCs (islet (2～ 10) × 103· per rabbit' per time × 2 to 13 times). The results of acute study showed that insulin in brain caused the strongest and longest hypoglyccmia and hyperinsulinemia acompanied by decrease of food and water intake.Atropine 0.5mg Partly alleviated above reactions. The nearly 2 years chronic graft study showed in 5 of 10 DM-rabbits the average blood glucose reduced more than 30% gradually and 3 of the 5 DM-rabbits completely recovered from hyperglycemia. The IRI in their brain was significantly higher than in normal rabbits. This suggests that inslllin as a neurotransansmitter in CNS,may participate in the regulation of peripheral insulin secretion and blood glucose level through the autonomic neurol fibres. Under long- term stimulation of insulin in over- physiologic level,the brain may amplify the peripheral effects of insulin through complex neuroendocrine network. It may also be possible that in this way CNS play an important role in the promoting the regeneration and restoration of islets.