裂褐菌木质素降解酶特性的研究

Study on Character of Lignin-Degradation Enzyme of Crack Brown Fungus

[目的]为木质素降解酶的大规模应用提供依据。[方法]以裂褐菌为材料,接种于固体PDA培养基和液体(天然与人工)培养基中,培养后测定固体培养基上菌落的直径,液体培养基中木质素过氧化物酶(Lip)和锰过氧化物酶(Mnp)的活性。[结果]裂褐菌接种到PDA培养基后,菌落在24 h内形成,在144 h内扩展到整个平板。30℃时生长较慢,菌落的扩展速度为0.296 1 mm/h,37℃条件下菌种萌发较早,菌落的扩展速度为0.443 3 mm/h。液体天然与人工培养基的Lip酶活在第8和10天达到最大,分别为373.09和278.75U/L;MnP酶活在第11和10天达到最大,分别为396和295.36 U/L。整个培养周期内,天然培养基的LiP和MP的产量均高于人工培养基。[结论]使用天然材料培养裂褐菌的效果优于人工材料。

[ Objective ] The aim was to provide the basis for large-scale application of liguin - degradation enzyme. [ Method ] With crack brown fungus as the material, it was inoculated on PDA solid medium and liquid （natural and artificial） medium. After culture, the diameter of the colony on solid medium and the activities of lignin peroxidase （Lip） and manganese peroxidase （MnP） in liquid medium were determined. [ Result] After the crack brown fungus was inoculated on solid PDA medium, the colony formed within 24 h and extended to the whole plate within 144 h. The crack brown fungus grew more slowly at 30 ℃ and the growth speed of colony was 0. 296 1 mm/h. The strain germinated earlier at 37 ℃ and the growth speed of colony was 0. 443 3 mm/h. The activity of Lip in natural and artificial liquid medium reached the highest at the 8th and 10th d after culture, being 373.09 and 278.75 U/L resp. while the activity MnP reached the highest at the llth and 10th d after culture, being 396 and 295.36 U/L resp. In the whole culture period, the yield of LiP and MnP in natural medium were all higher than that in artificial medium. [ Conclusion ] The effect of culturing crack brown fungus with natural medium was better than that with artificial medium.