小鼠肠道菌群失调动物模型的制备

Establishment of Experimental Animal Model of Intestinal Microflora Dysbiosis

用乳糖酸红霉素(2.94g/kg)、头孢氨芐(6.19g/kg)、盐酸林可霉素(2.02g/kg)、氨芐西林钠(5.5g/kg),3次/天,连续10天灌服小鼠,分别于灌服后第2d、4d、6d、8d、10d、12d、14d、16d对直肠和盲肠粪便中杆菌和球菌的菌落数进行测定。结果表明:灌服抗生素后杆菌菌落数量减少,球菌菌落数量增加,取代杆菌成为直肠和盲肠中的优势菌群;停药后第6d,除盐酸林可霉素组外,其它灌服组杆菌和球菌数量均可逐渐恢复。结论:乳糖酸红霉素、头孢氨芐、盐酸林可霉素及氨芐西林钠均能导致小鼠肠道菌群失调,其中盐酸林可霉素对肠道杆菌的抑制作用较明显,诱导效果较稳定和持久。

To establish a mice model of gastrointestinal microflora imbalance, the mice were orally inoculated with Erythromycin Laetobionatefor Injection （2.94g/kg） , Cefalexin （6.19g/kg）, Lincomycin Hydrochloride Injection （2.02g/kg） and Ampicillin Capsules （5.5g/kg） there times each day for 10 days. The colony of bacillus and coccus in rectum and cecum were detected every- day between 2 and 16 days postinoculation （p. i. ）. The Results show that during the term of inocu- lation, the colony of coccus in rectum and cecum gradually increased and become the prominent bac- teria in the intestine. By the 16 days p. i. , the levels of bacillus and coccus recovered gradually and near to the control mice except the lactic acid group. Finally, the Conclusions were drawn below： the results suggest that four antibiotics can stably induce intestinal microflora dysbiosis in mice. Inhibiting effect of Lincomycin Hydrochloride on bacillus is very stable and lasting than others.