苯丁酸钠对甲状腺滤泡癌细胞侵袭能力及MMP-9和TIMP-1表达的影响

Effects of sodium phenylbutyrate on invasive ability of human thyroid follicular carcinoma cell line and expression of MMP-9 and TIMP-1

目的:探讨苯丁酸钠(sodium phenylbutyrate,NaBP)对甲状腺滤泡癌细胞CGTHW-1的侵袭能力及基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)和金属蛋白酶组织抑制剂-1(tissue inhibitor of metalloproteinase,TIMP-1)表达的影响。方法:培养CGTHW-1细胞,通过Transwell侵袭实验观察苯丁酸钠对CGTHW-1细胞侵袭能力的影响,采用免疫细胞化学SP法及RT-PCR观察苯丁酸钠对CGTHW-1细胞中MMP-9和TIMP-1蛋白及mRNA表达的影响。结果:4 mmol/L苯丁酸钠作72 h,CGTHW-1细胞的侵袭细胞数显著减少[(29.8±1.77)vs(11.00±2.59),(P<0.01)]。免疫细胞化学和RT-PCR检测结果显示,4、6 mmol/L苯丁酸钠显著抑制CGTHW-1细胞中MMP-9、TIMP-1蛋白及mRNA的表达(P<0.05)。结论:苯丁酸钠可通过下调MMP-9和TIMP-1的表达进而降低甲状腺滤泡癌CGTHW-1细胞的侵袭能力。

Objective： To investigate the effects of sodium phenylbutyrate （ NaPB ） on the matrix metalloproteinase-9 （MMP-9） and tissue inhibitor of metalloproteinase-1 （ TIMP-1 ） expression and invasive ability of human thyroid follicular carcinoma cell line CGTHW-1. Methods： CGTHW-1 cells were treated with different concentrations of NaPB, then the invasive ability of CGTHW-1 cells was assessed using Transwell assay. The expression of MMP-9 and TIMP-1 was exam- ined by immunocytochemistry staining and RT-PCR in CGTHW-1 cells. Results： After treatment with NaPB （4 mmol/L） for 72 h, CGTHW-1 cells passing the Transwell were significantly reduced [ （ 29.8±1.77 ） vs （ 11.00 ± 2.59 ）, P 〈 0. 05 ]. Immunocytochemistry staining and RT-PCR demonstrated that protein and mRNA expression of MMP-9 and TIMP- 1 were significantly down-regulated in CGTHW-1 cells after NaPB treatment （4, 6 mmol/L ） （all P 〈 0. 05 ）. Conclusion： NaPB can inhibit CGTHW-1 cell invasion by down-regulating MMP-9 and TIMP-1 expression.