乳腺癌中肾上腺髓质素的表达及其与癌组织分化、转移间的关系

Expression of adrenomedullin in breast cancer tissues and its relationship with differentiation and metastasis of breast cancer

目的:探讨乳腺癌组织中肾上腺髓质素(adrenomedullin,ADM)的表达情况及其临床意义。方法:取河北医科大学第四医院外一科2005年3月至2005年4月手术切除的32例乳腺癌组织,以及同组病例距癌组织外缘5 cm外的癌旁乳腺组织,采用RT-PCR检测人乳腺癌细胞株MCF-7和32例乳腺癌组织及癌旁组织中ADM mRNA的表达情况,免疫组织化学染色检测32例乳腺癌组织中ER、PR、C-erbB-2的表达情况,并分析ADM mRNA与临床病理指标之间以及与ER、PR、GerbB-2表达间的关系。结果:人乳腺癌MCF-7细胞株呈ADM mRNA阳性表达。乳腺癌组织中ADM mRNA阳性表达率为75%(24/32),显著高于癌旁5 cm外乳腺组织(0%,0/32;P<0.01)。人乳腺癌组织ADMmRNA的表达与肿瘤大小、TNM分期、病理类型、ER、PR和C-erbB-2等临床病理指标无关,但与组织学分级(P<0.01)和腋窝淋巴结转移有关(P<0.05)。结论:ADM mRNA在乳腺癌组织中表达增加,其强阳性表达与组织学分化差或腋窝淋巴结转移密切相关。

Objective：To investigate the expression of adrenomedullin （ADM） in breast cancer tissues and its relationship with differentialtion and metastasis of breast cancer. Methods ： RT-PCR was used to examine ADM mRNA expression in 32 breast cancer tissues （the patients were treated in the Fourth Affiliated Hospital of Hebei Medical University from March 2005 to April 2005 ） and the corresponding adjacent tissues （5 cm away from tumors） , as well as in MCF-7 cell line. Expressions of ER, PR and C-erbB-2 in breast cancer were examined by immunohistochemical staining. Results： Human breast cancer cell line MCF-7 was positive of ADM. ADM mRNA expression was detected in 75% （24/32） breast cancer samples, which was significantly higher than that in the corresponding adjacent tissues （0% , 0/32, P 〈 0.01 ）. We found that ADM expression was correlated with the differentialtion degree and lymph node metastasis of breast cancer （P 〈0.01 and P 〈0.05, respectively）, and was not correlated with the expression of EP, PR or C-erbB-2, the tumor size, TNM staging, or the pathological types. Conclusion： The expression of ADM is increased in breast cancer tissues, which is correlated with the poor differentiation and lymph node metastasis of the breast cancer.