膀胱尿路上皮癌中CD24、VEGF、MVD的表达及其临床意义

Clinical significance of CD24,VEGF,MVD expression in urothelial carcinoma of the bladder

目的探讨CD24和血管内皮生长因子(VEGF)在膀胱尿路上皮癌中的表达及与肿瘤临床特征的关系。方法采用免疫组织化学SP法检测了120例(80例经尿道切除标本,40例根治性膀胱切除标本)膀胱尿路上皮癌组织,根据VEGF的染色情况计算出膀胱尿路上皮癌组织的微血管密度(MVD)。结果在正常上皮中,CD24局限于细胞浆表达的极弱。膀胱尿路上皮癌组织中CD24的阳性表达率明显高于正常膀胱上皮。高肿瘤分级和高pTNM分期、淋巴结转移的癌组织中CD24的阳性表达率显著高于低肿瘤分级和低pTNM分期、无淋巴结转移的癌组织(P<0.05)。膀胱尿路上皮癌组织中CD24阳性表达率与MVD呈正相关(r=0.243,P<0.05)。结论CD24在膀胱尿路上皮癌中表达明显上调,与膀胱尿路上皮癌临床分期、病理分级呈正相关,与肿瘤的浸润、转移、血管形成密切相关。

Objective To investigate the clinical significance of CD24 and vascular endothelial growth factor （VEGF） expression in urothelial carcinoma of the bladder. Method SP immunohistochemistry was used to detect expression of CD24 in 120 specimens （80 transurethral resection, 40 radical cystectomy） of bladder urothelial carcinoma. Microvessel density （MVD） was calculated according to the positive staining for VEGF. Results In normal urothelium, CD24 was localized to the cytoplasm of cell with very low intensity, and CD24 expression was higher in bladder urothelial carcinoma than in the normal urothelium. The higher expression of CD24 was significantly correlated with higher tumor grade, higher pTNM stage, and lymph node metastasis （P〈0.05）. There was a significant correlation between the positive rate of CD24 expression and the value of MVD in bladder urothelial carcinoma MVD （r= 0. 243, P 〈 0. 05）. Conclusion CD24 expression is obviously upregulated in urothelial carcinoma, which has not only a positive correlation with the grade and stage of tumor, but also correlation with the tumor invasion, metastasis and angiogenesis.