PARP-1和caspase-3在结直肠肿瘤中的表达和意义

Expressions and Significance of PARP-1 and Caspase-3 in Colorectal Neoplasms

背景：聚腺苷二磷酸核糖聚合酶-1（PARP-1）主要参与对DNA损伤的反应,caspase-3主要参与细胞凋亡的调控,两者与结直肠癌发生的关系尚不十分清楚。目的：探讨PARP-1和caspase-3在结直肠腺瘤和腺癌中的表达及其与肿瘤临床病理特征的关系。方法：以免疫组化方法检测59例结直肠腺瘤、70例结直肠腺癌和10例癌旁黏膜石蜡包埋标本中PARP-1和caspase-3的表达,分析腺瘤和腺癌组织中两者表达与肿瘤临床病理参数的相关性,以及两者间的相关性。结果：结直肠腺瘤和腺癌组织中,PARP-1的阳性表达率显著高于癌旁结直肠黏膜（55.9%和82.9%对20.0%,P〈0.05）,caspase-3的阳性表达率显著低于癌旁结直肠黏膜（33.9%和28.6%对100.0%,P〈0.05）。结直肠腺瘤中,PARP-1的表达与腺瘤数目相关,caspase-3的表达与上皮内瘤变程度相关;结直肠腺癌中,PARP-1的表达与癌组织分化程度相关。PARP-1与caspase-3在结直肠腺瘤和腺癌组织中的表达无相关性。结论：PARP-1和caspase-3在结直肠腺癌的发生、发展过程中起有重要作用,两者联合检测对结直肠腺癌的早期诊断具有一定价值。

Background： Poly（ADP-ribose） polymerase-1 （PARP-1） is involved in cellular responses to DNA damage, while caspase-3 mainly regulates cell apoptosis. The relevance of PARP-1, caspase-3 and colorectal cancer is still uncertain. Aims： To investigate the expressions of PARP-1 and caspase-3 in colorectal adenoma and adenocarcinoma, and their relationships with the clinicopathologic characteristics of colorectal neoplasms. Methods： PARP-1 and caspase-3 expressions were determined immunohistochemically on paraffin-embedded specimens of 59 colorectal adenoma, 70 colorectal adenocarcinoma and 10 paracancerous tissues. Relationships between expressions of PARP-1 and caspase-3 and the clinicopathologic features of colorectal adenoma and adenocarcinoma, as well as the relevance of PARP-1 and caspase-3 in colorectal adenoma and adenocarcinoma were analyzed. Results： PARP-1 immunoreactivity was significantly higher in colorectal adenoma and adenocarcinoma than in paracancerous mucosa （55.9% and 82.9% vs. 20.0%, P〈0.05）. In contrast, caspase-3 expression was significantly lower in colorectal adenoma and adenocarcinoma than in paracancerous mucosa （33.9% and 28.6% vs. 100.0%, P〈0.05）. PARP-1 expression was significantly correlated with the number of adenoma and the differentiation of adenocarcinoma, while caspase-3 expression was significantly correlated with the degree of intraepithelial neoplasia of adenoma. PARP-1 expression was not correlated with caspase-3 expression in colorectal adenoma and adenocarcinoma. Conclusions： Both PARP-1 and caspase-3 may be involved in the pathogenesis and development of colorectal adenocarcinoma. Combined detection of these two markers may be valuable for the early diagnosis of colorectal adenocarcinoma.