小油桐种子的^60Co-γ射线辐射敏感性及半致死剂量的研究

Sensitivity of Jatropha curcas seeds to ^(60)Co-γ radiation and their medial lethal doses in radiation breeding

目的研究3个不同产地小油桐种子对60Co-γ射线辐射的敏感性,确定其辐射处理的半致死剂量。方法以种子50%发芽率为基准,通过直线回归方程计算3个不同种源的小油桐种子60Co-γ射线辐射的半致死剂量。结果和结论3个不同种源的小油桐种子的辐射半致死剂量分别为178Gy(广东)、132Gy(海南)、198Gy(印度)。60Co-γ射线辐射可引起幼苗某些表型发生改变,随着辐射剂量的增加,小油桐叶片形状发生多种变异。研究结果可作为重要药用及能源植物小油桐辐射诱变育种的参考依据。

Objective To study the sensitivity of Jatropha curcas seeds from three different locations to ^60Co-γ radiation and to determine the medial lethal doses （LD50） of ^60Co-γ radiation for these seeds. Methods Six different radiation doses （0, 100, 150, 200, 250 and 300 Gy） were used. Based on the germination rate 50%, LD50 doses of ^60Co-γ radiation for the seeds were calculated using linear regression equation. Results LD50 doses of ^60Co-γ radiation for these seeds were 178 Gy （seeds from Guangdong）, 132 Gy （seeds from Hainan） and 198 Gy （seeds from India） respectively. Increasing radiation doses caused more significant changes in leaf shape of the M1 seedlings. Conclusion The results provides an important experimental basis for the radiation breeding of the important herbal and energy plant J. curcas.