TRP亚家族mRNA在大鼠睾丸组织中的表达

Expression of transient receptor potential subfamily mRNAs in rat testes

目的探讨瞬时受体电位(TRP)基因亚家族TRPV、TRPM家族mRNA在大鼠睾丸组织中的表达。方法选用SD大鼠,处死后取其睾丸用常规RT-PCR检测TRPV和TRPM亚家族mRNA的表达。结果TRPV4、TRPV5、TRPV6、TRPM3、TRPM4与TRPM8在睾丸有表达,其他TRPV和TRPM家族成员未见有表达。结论TRPV4、TRPV5、TRPV6、TRPM3、TRPM4与TRPM8在睾丸组织上有表达,这为睾丸组织上TRPV和TRPM功能的研究奠定了基础,为进一步探讨TRP家族与睾丸相关疾病之间关系奠定基础。

Objective To study the expression of the mRNAs of transient receptor potential （TRP） gene subfamily TRPV and TRPM in rat testes. Methods Normal SD rat testes were collected and the expression of TRPV and TRPM mRNAs were detected by routine RT-PCR. Results The TRPV4, TRPV5, TRPV6, TRPM3, TRPM4 and TRPM8 mRNAs were detected in the rat testes, but the other members of TRPV and TRPM family were not detected. Conclusions TRPV4, TRPV5, TRPV6, TRPM3, TRPM4 and TRPM8 are expressed in rat testes. This finding provides the basis for exploring the functions of TRPV and TRPM in the testes and the relation between testis diseases and the TRP family.