抑制素基因免疫对南阳黄牛生殖激素的影响

Effects of Inhibin Gene Immunization on Reproductive Hormone Secretions in Nanyang Yellow Cattle

选择40头南阳黄牛,用3.0 mg抑制素基因重组质粒(pCIS,免疫组,n=30)或同剂量的生理盐水(对照组,n=10)间隔21 d注射2次,分别在首次免疫当天、首次免疫后第10天和第21天、加强免疫后第10天和第45天收集血清,应用放射免疫法(RIA)测定处理后不同时期血清中激素水平。结果表明:在5个采血时间点内,加强免疫后第10天抗体阳性牛比率最高,为37.5%,与其他采血时间点有显著差异。免疫组促卵泡素(FSH)平均含量比对照组高,但差异不显著;17-β雌二醇(E2)和孕酮(P4)水平在加强免疫后2组有显著性差异。另外,抑制素抗体阳性牛的FSH、E2和P4比阴性牛高,但差异不显著。这些结果说明抑制素基因重组质粒可促进肉牛产生特异性抗体,且对FSH、E2和P4水平有影响。

A total of 40 Nanyang Yellow cattle heifers were given double injections of either 3.0 mg inhibin eukaryotic expressing plasmid （pCIS, n= 30 as an immunized group） or an equal volume of physio,10,21 after the primary immunization, and on day 10 and 45 after the booster immunization. Plasma concentrations of follicle stimulating hormone （FSH）, 17β-estradiol （E2） and progesterone （P4） were measured by a radioimmunoassay （RIA）. The results showed that the highest percentage of the cattle with positive antibodies against inhibin was 37.5%,which was observed on the 10th day after booster immunization, the percentage of positive antibodies on day 10 was significantly different from those on the other days （P〈0.05）. In addition, FSH level in the immunized group was a little higher than that in the control group （P〉0.05） with no significant difference. the difference was significant for both 17 β- estradiol （E2） and progesterone （P4） levels after the booster immunization of the tested group and the control. In addition,there is no difference for the mentioned three hormones in the positive antibodv cattle in contrast to the negative antibody ones. All the results demonstrate that the recombined plasmid of inhibin gene can stimulate yellow cattle to produce antibodies against inhibin as well as have an effect on FSH,E2 and P4 content.