维拉帕米及尼卡地平对瘢痕成纤维细胞胶原合成的影响

The Effect of Verapamil and Nicardipine on Fibroblast Collagen Synthesis of Scar

目的:研究钙离子拮抗剂维拉帕米及尼卡地平对体外培养的瘢痕成纤维细胞胶原合成的影响,同时对两种药物疗效进行比较,讨论两种药物的作用特点。方法:向对数生长期的增生性瘢痕成纤维细胞及瘢痕疙瘩成纤维细胞中加入不同浓度的两种药液,培养48小时后进行羟脯氨酸比色试验,并将OD值依公式换算成羟脯氨酸含量,进行统计学比较。结果:维拉帕米及尼卡地平组随着用药浓度的提高,细胞形态变化越来越明显,两种成纤维细胞显示出类似的形态学特点;与对照组比较,两药均可以降低培养上清液中羟脯氨酸的含量,且随用药浓度增加,羟脯氨酸含量进一步下降,高(100μmol/L)低(10μmol/L)浓度组均有显著性差异(p<0.05),同时两药作用特点也有所不同。结论:维拉帕米和尼卡地平均可降低增生性瘢痕及瘢痕疙瘩的胶原含量,抑制胶原合成,对于增生性瘢痕,尼卡地平抑制作用略强于维拉帕米,而对于瘢痕疙瘩,则维拉帕米的抑制作用略强于尼卡地平。

Objective： To study the effect of verapamil and nicardipine on fibroblast collagen synthesis in vitro culture, to compare with the tratment effects and to discuss the the characteristics of role of the two drugs. Method： Adding dividedly the solution of two drugs with different concentration into the heperplastic scar fibroblasts and keloid fibroblast which were in logarithmic growth phase. After 48 hours in vitro culture, hydroxyprohne colorimetric test was conducted, then statistics comparison was carried out in accordance with hydroxyproline content which was calculated based on OD value. Result： Following the increasing of concentration of verapamil and nicardipine, the cell shape changed obviously. Both two drugs can reduce the contents of hydroxyproline in the supematant of cultured hypertrophic scar fibroblast （HSFB） and keloid fibroblast （KDFB）. The hydroxyproline content in the high concentration group （100umol/1） and the low concentration group （10umol/1） showed significant difference （p 〈 0. 05 ）. Conclusion： Verapamil and nicardipine can reduce the concent of collagen of HSFB and KDFB and inhibit collagen synthesis simultaneously. For hypertrophic sears, nicardipine inhibition was slightly stronger than verapamil, while, for the keloid, the inhibitory effect of verapamil slightly stronger than nicardipine.