运用均匀设计法优化超声波破碎根结线虫天敌真菌-1细胞的条件研究

Using Uninform Design to Optimize Ultrasonic Cell-break Conditions of DFRKN-1

运用均匀设计法对超声波破碎根结线虫天敌真菌-1(destroying fungi of root-knotnematode以下简称DFRKN-1)细胞提取碱性磷酸酶的条件进行优化,摸索出了超声波破碎条件为:超声功率100 W,菌液浓度30%,超声时间2 s,间歇时间7 s,总时间35 min时,得到细胞破碎混合物中蛋白浓度最高,蛋白浓度从0.7306 g/mL提高到了2.6269 g/mL,比优化前提高了3.6倍;超声功率100 W,菌液浓度30%,超声时间6.5 s,间歇时间7 s,总时间为10 min时,得到细胞破碎混合物中碱性磷酸酶酶活力最高,从458 U/mL提高到1 725 U/mL,比优化前提高了3.8倍。

Using the uniform design to optimize ultrasonic cell-break conditions of DFRKN -1 to isolate alkaline phosphatase, and find out the ultrasonic cell-break condition was： When supersonic power 100 W, consistency 30%, supersonic time 2 s, intermittence time 7 s, total time 35 min, obtains the consistency of protein was highest in the cell mixture, the consistency of protein enhanced from 0. 7306 g/mL to2. 087 g/mL, compared before the optimization to enhance 2. 86 times; When supersonic power 100 W, consistency 30M, supersonic time 6. 5 s, intermittence time 7 s, the total time 10 min, obtains the alkalinity phosphatase enzyme activity was highest in the cell mixture, enhances from 458 U/mL to 1725 U/mL, compared before the optimization to enhance 3. 8 times.