摘要
以藤茶初春萌发的幼嫩枝条为外植体,以MS培养基为基本培养基,研究不同植物生长调节物质、不同碳源和不同碳源浓度对藤茶愈伤组织的诱导效应。结果表明:茎和藤须为外植体,其愈伤组织诱导率较高,且诱导愈伤组织的最适培养基为:MS+6-BA 1.5 mg/L+NAA 0.2mg/L+PVP 2 g/L。
This article used the young tender branch of Amplelopsis grossedentata 'Lvfeng'which sprouted at the beginning of spring as explant, studied on callus induction from the MS basic culture medium,which were added different plant growth regulator, different carbon sources or the different concentration of carbon. The results showed that the stem and its stem tendril had the best effect induced and the most suitable induction culture medium was MS+6-BA 1.5 mg/L+ NAA 0. 2 mg/L+PVP 2 g/L.
出处
《北方园艺》
CAS
北大核心
2009年第3期59-61,共3页
Northern Horticulture
基金
国家民委科研资助项目(08HB04)
湖北民族学院团队资助项目
关键词
绿凤藤茶
愈伤组织
诱导
Amplelopsis grossedentata Lvfeng
Callus
Induction