绿凤藤茶愈伤组织诱导的初步研究

Preliminary Study on Callus Induction of Amplelopsis grossedentata 'Lvfeng'

以藤茶初春萌发的幼嫩枝条为外植体,以MS培养基为基本培养基,研究不同植物生长调节物质、不同碳源和不同碳源浓度对藤茶愈伤组织的诱导效应。结果表明:茎和藤须为外植体,其愈伤组织诱导率较高,且诱导愈伤组织的最适培养基为:MS+6-BA 1.5 mg/L+NAA 0.2mg/L+PVP 2 g/L。

This article used the young tender branch of Amplelopsis grossedentata ＇Lvfeng＇which sprouted at the beginning of spring as explant, studied on callus induction from the MS basic culture medium,which were added different plant growth regulator, different carbon sources or the different concentration of carbon. The results showed that the stem and its stem tendril had the best effect induced and the most suitable induction culture medium was MS＋6-BA 1.5 mg/L＋ NAA 0. 2 mg/L＋PVP 2 g/L.