合果芋组织培养快繁技术研究

In vitro Culture and Rapid Propagation of Syngonium Podophyllum Schott

以合果芋幼嫩侧芽为外植体,研究了不同激素浓度对合果芋组织培养的影响。结果表明:用0.1%的氯化汞溶液+3滴Tween20对外植体处理35 min,灭菌效成功率达75%;较好的诱导培养基为MS+BA 5.0 mg/L+IAA 0.2 mg/L,最佳的增殖培养基为BA 2.0 mg/L+NAA0.1 mg/L,最好的生根培养基为MS+NAA 0.3 mg/L+IAA 0.2 mg/L;生根瓶苗置于室外自然光线下练苗7 d后,移栽到珍珠岩∶腐殖土∶壤土=1∶1∶1的基质中,成活率可达94%以上。

The lateral tender buds of Syngonium Podophyllum Schott as explains were used. Effect on tissue culture of MS containing different content BA, NAA and IAA were studied. The results indicated that the rate of survived buds could approach to 75% when they were sterilized for 35 min in 0. 1% HgCl2 ＋3 drops Tween20. The medium MS＋BA 5. 0 rng/L＋IAA 0. 2 mg/L was suitable for introduction of callus or adventitious buds. The medium BA 2. 0 rag/L＋ NAA 0. 1 mg/L was the best for multiplication. The optimum rooting medium was MS＋NAA 0. 3 mg/L＋IAA 0. 2 mg/L After the rooting plantlets in vitro were treated for 7 d in natural light, they were transplant in medium containing humus, perlite and soil （1 ： 1 ： 1）, the living rate of the plantlets could reach 94%.