摘要
以新发现的大丽菊花色突变体为材料,选取10个具有10个碱基长度的随机引物,对5种突变个体的基因组DNA进行了RAPD分析。结果表明：10个随机引物共扩增出152条DNA条带,分子量大小在250-1 600 bp之间,其中有4个引物的扩增结果中检测到了差异性条带,在DNA水平上初步证实了5种花色突变体是由于遗传因素导致的稳定变异。
The RAPD analysis of genomic DNA, generated by 10 random primers, 10 nt in every one, discrinainated 5 flower color mutants of novel versicolor mutants in Dahlia pinnata Cav. The results showed that 152 bands were amplified in 10 primers, and each molecular weights range from 250-1 600 bp. Among them particular bands were produced in 4 primers, which suggested primarily in the DNA level that 5 flower color mutants varied due to the genetic factors.
出处
《北方园艺》
CAS
北大核心
2009年第3期75-77,共3页
Northern Horticulture
关键词
大丽菊
花色突变体
随机扩增多态性DNA
Dahlia pinnata Cav
Versicolor mutant
Random amplified polymorphic DNA
