逍遥散诱导乳腺癌细胞凋亡实验研究

Study on Apoptosis Induction by Ease Powder in Human Mammary Cancer Cell Line

目的:研究逍遥散提取液诱导乳腺癌细胞凋亡的作用。方法:应用形态学观察和流式细胞术等方法对逍遥散提取液处理的人乳腺癌(MCF-7)细胞进行了检测和观察,并运用caspase-3抑制剂检测凋亡。结果:逍遥散提取液处理后24h细胞具有典型的凋亡形态特征;用流式细胞仪测定细胞周期,可见明显的凋亡峰;caspase-3抑制剂阻碍凋亡的发生。结论:逍遥散提取液可诱导人乳腺癌(MCF-7)细胞发生凋亡,其诱导细胞凋亡的机制可能部分是通过Caspase-3途径。

Objective： To study Ease powder aquous-induced apoptosis on human mammary cancer cells. Methods ： cell morphology and flow cytometry were used to examine human mammary cancer cell line MCF-7 that treated with Ease powder aquous, and make use of the depressants of caspase-3 to apoptosis examination. Results ： When cells were treated with Ease powder aquous for 48h,The cell displayed apoptosis remarkably in morphology changes; DNA histograms by flow cytometric analysis displayed distinct apeptotic peak; the depressants of caspase-3 inhibit the occurrence of apoptosis. Conclusion ： The effect of Ease powder aquous on human mammary cancer cells resulted from induced apoptosis, and Ease powder aquous induced apoptosis is partially dependent on caspase-3 pathway in MCF-7.