摘要
目的:采用HPLC法测定六味地黄丸(浓缩丸)中丹皮酚的含量。方法:以十八烷基硅烷键合硅胶为填充剂;甲醇-水(70:30)为流动相;检测波长为274nm;流速0.8ml/min;柱温:室温。结果:丹皮酚含量在8.4~33.6μg/ml范围内具有良好线性关系,r=0.99999,平均加样回收率为99.32%,RSD=1.2%(n=9)。结论:结果表明,该方法方便快捷、准确、灵敏度高、重现性好。
Objectives: To determine Deonol Contert in Liuweidihuang Pill HPLC. Method: 18 alkyl silane bonded silica to fill in the blank;methanol-water (70:30)for the mobile phase detection at 274nm; flow rate:0. 8ml/min ; column:room temperature. Result: paeonol content of 8.4-33.6μg/ml within the framework of a linear, r=0. 99999, average recovery of 99. 32%, RSD=1. 2%(n=9). Conclusion: The results show that the convenient and fast, accurate, sensitive and reproducible.
出处
《黑龙江医药》
CAS
2009年第2期120-122,共3页
Heilongjiang Medicine journal