HPLC法测定清开灵口服液中绿原酸的含量

Determination of Chlorogenic Acid in Qingkailing Oral Liquid by HPLC

目的:建立用HPLC法测定清开灵口服液中绿原酸的含量。方法:采用日本岛津SHIMADZU LC-2010A型液相色谱仪,ULTIMATE AQ-C18色谱柱,检测波长327nm,以甲醇-0.2mol/L磷酸二氢钠(调pH至3.2)(15:85)为流动相,流速为0.8ml/mi n。结果:精密度及回收率结果良好,平均回收率为99.67%(n=10),RSD=1.30%。结论:方法准确可靠,可用于清开灵口服液中绿原酸的含量测定。

Objective： To build up an HPLC method for determination of chlorogenic acid content in Qingkailing oral liquid. Methods：The HPLC with ULTIMATE AQ- C18was used and detection wavelength was 327nm and a mixure of methanol-sodium dihydrogen phosphate solution （0.2mol/L, PH3.2 ） （15：85） as mobile phase. The flow rate was 0. 8ml/min. Result：The average recovery was 99.67%,and RSD was 1.30%. Conclusion：This method is reliable, accurate and suitable for the determination of chlorogenic acid in Qingkailing oral liquid .