头针对局灶性脑缺血再灌注模型大鼠治疗时间窗研究

The Research of Head Acupuncture at Different Therapeutic Time Windows on Neuroprotection in Focal Cerebral Ischemia/Reperfusion Injury in Rats

目的:探讨针刺百会透曲鬓对脑缺血再灌注模型(MCAO/R)大鼠脑保护作用的最佳治疗时机。方法:采用线栓法复制MCAO/R模型,于再灌注后2h、6h、12h、24h、48h5个不同时间窗开始针刺,比较术后7天MCAO/R模型大鼠的神经功能评分以及脑梗死体积的变化。结果:各针刺组的神经功能评分、脑梗死体积与模型组比较均有显著差异(P<0.05)。各针刺组间,神经功能缺损评分比较,48hR针刺组与其余四组存在显著性差异(P<0.05),而该四组间无统计学差异(P>0.05)。脑梗死体积测定表明,2hR、6hR针刺组脑梗死体积最小,且两组无明显差异(P>0.05);12hR针刺组脑梗死体积比2hR、6hR针刺组明显增大(P<0.05);而24hR、48hR针刺组脑梗死体积最大,与前三组比较有统计学差异(P<0.05)。结论:针刺百会透曲鬓对MCAO/R模型大鼠具有脑保护作用,并且超早期治疗更有意义。

Objective:To study the selecting therapeutic time of head acupuncure(HA) on neuroprotection in focal cerebral ischemia/reperfusion injury in rats.Method:Forty-eight rats were randomized into model(n=8),HA-2hR(n=8),HA-6hR(n=8),HA-12hR(n=8),HA-24hR(n=8)and HA-48hR(n=8)groups.The model was induced by middle cerebral artery occlusion/reperfusion(MCAO/R)with suture method.HA was delivered to'Baihui'and'Qubin' through acupuncture needles 2hR,6hR,12hR,24Hr and 48hR respectively following MCAO/R,twice daily,continuously for 7 days.Functional neurological outcome was determined 1h and 7d after reperfusion.Infact volume was measured 7d after reperfusion.Results:In the HA groups,functional neurological outcomes and infact volumes were degraded distinctly in comparison with model group(P<0.05).There was an evident differences between HA-48hR group and other four groups in functional neurological outcomes(P<0.05).Among the HA groups,infact volumes of HA-2hR and HA-6hR were the most largest,while the infact volumes of HA-24hR and HA-48hR were the most smallest.Conclusion:HA reduced infarct size,improved functional neurological outcome and played the function of neuroprotection.When acupuncture treatment was administered in the early period of acute cerebral ischemia,the substantial significance could be found.