嗜酸乳杆菌高密度发酵条件的研究

Study on High Cell Density Culture of Lactobacillus acidophilus

主要研究了嗜酸乳杆菌生长繁殖的环境条件(温度、接种量、起始pH等)和培养基组成对其影响。优化后确定了嗜酸乳杆菌的高密度培养条件为:起始pH值为5.8,培养温度为37℃,培养条件为亨-盖特厌氧培养,接种量为4%,培养时间为22 h。并且筛选了碳源,氮源及氮源的浓度。基配比为:2%乳清粉(w/v),1.5%牛肉膏(w/v),1.5%蛋白胨(w/v),0.058%硫酸镁(w/v),0.025%硫酸锰(w/v),0.22%乙酸钠(w/v),0.2%磷酸氢二钾(w/v)。将该菌在此增菌培养基上培养,在37℃下培养22 h,菌数可达到1.17×1011 cfu/mL。

This paper mainly studied the high cell density culture of Lactobacillus acidophilus ,and discussed the effects of cultural condition （temperature,inoculation capacity and initial pH）and media composition. The optimal culturing conditions were：pH5.8,cuhure temperature 37℃ ,4% inoculation capacity;culture composition of whey mist 2% ,beef extract 1.5% ,peptone 1.5% ,com,MgSO4 0.058% ,MnSO4 0.025% ,NaAC 0.022% ,K2HPO4 0.2% .The amount of lactobacillus acidophilus was up to 1.17×10^11CFU/mL for 22 hours based on the above conditions.